Ansoumane Kourouma
Huazhong University of Science and Technology, China
Title: The effects of 4-nonylphenol administration induced oxidative damages in liver and contributes to hepatic steatosis in male rats
Biography
Biography: Ansoumane Kourouma
Abstract
An emerging literature suggests that early life exposure to 4-nonylphenol (4-NP), a widespread endocrine disrupting chemical, may increase the risk of metabolic syndrome. In this study, we investigated the hypothesis that intraperitoneal administration of 4-NP induce hepatic steatosis in rat. 24 males Sprague-Dawley rats were administered with 4-NP (0, 2, 10 and 50 mg/kg b.wt) in corn oil during 30 days. Liver histology, biochemical analysis and gene expression profiling were examined. After treatment, abnormal liver morphology and function were observed in the 4-NP-treated rat, and significant changes in gene expression as indicator of hepatic steatosis and apoptosis were observed compared with controls. Up-regulated genes involved in apoptosis, Hepatotoxity and OS, increased ROS and decrease of antioxidant enzyme were observed in the 4-NP exposed rat. Extensive fatty accumulation in liver section and elevated serum SGOT, SGPT, LDH and γ-GT were also observed. Incidence and severity of liver steatosis was scored and taken in to consideration (steatosis, ballooning and lobular inflammation). Hepatocyte apoptosis could promote NAFLD progression; Fas/FasL, TNF-α and Caspase-9 mRNA activation were important contributing factors to hepatic steatosis. These findings provide the first evidence that 4-NP affects the gene expression related to liver hepatotoxicity, which is correlated with hepatic steatosis.