Mahmoud M Zakaria Ibrahim
Mansoura University, Egypt
Title: Anticancer activity of Aloe vera and Calligonum comosum extracts separately on hepatocellular carcinoma cells
Biography
Biography: Mahmoud M Zakaria Ibrahim
Abstract
Objective: The present study aimed to investigate the in vitro anticancer effect of Aloe vera and Calligonum comosum extracts against hepatocellular carcinoma (HepG2) cells. Methods: HepG2 cells were tested against different doses of A. vera and C. comosum. Viability of the cells was assessed by MTT assay. Evaluation of apoptosis and DNA damage in HepG2 cells were performed using Annexin V Apoptosis Detection Kit. The expression of p53 and anti-apoptotic (Bcl-2) were tested by Real Time-PCR and flow cytometer analyzer. Hematoxylin & Eosin stained sections from untreated and treated HepG2 cells were observed using light microscopy. Results: The IC50 values of A. vera and C. comosum extracts were (10.45±0.31) and (9.6±0.01) µg/ml respectively. The extracts separately increased cytotoxicity against HepG2 cells in a time and dose dependent manners. Also, it apparently induced apoptosis through increase P53 and decrease Bcl-2 genes expressions. Conclusion: The results indicated that the extracts could have anti-hepatocarcinogenic effect at least in part through modulation of apoptosis.